Peptide Research Protocols. Endothelin
Janet J. Maguire, Anthony P. Davenport
A panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of the endothelin system from its synthesis and metabolism, to its function in health and disease. Theses methods use state-of-the-art molecular techniques to quantify the expression of mRNA for both endothelin receptors and the endothelin converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function.
類別:
年:
2010
版本:
1st Edition.
出版商:
Humana Press
語言:
english
頁數:
242
ISBN 10:
1617372919
ISBN 13:
9781617372919
系列:
Methods in Molecular Biology
文件:
RAR, 1.92 MB
IPFS:
,
english, 2010